Use of Ultivue InSituPlex®Multiplex Immunofluorescence to Localize and Quantify Regulatory T Lymphocytes in Crohn’s Disease and Ulcerative Colitis

A high-plex mIF workflow was used to identify and quantify conventional Treg and cytotoxic T cells in Crohn’s disease and ulcerative colitis. Using a 5-marker InSituPlex® panel, the study mapped immune populations directly in FFPE GIT tissue, revealing distinct Treg/CTL patterns in small intestine versus colon across IBD and controls.