Sequential same slide multiplex immunofluorescence and H&E staining for combined phenotypic and morphologic characterization of formalin-fixed paraffin-embedded tissue sections

A sequential same-slide workflow supports multiplex immunofluorescence and H&E imaging on a single FFPE section, preserving morphology while enabling clear tumor–stroma classification, immune phenotyping, and PD-L1 assessment from the same tissue slice.