Measuring concordance of CD8 and PD-L1 expression in Non-Small Cell Lung cancer between a novel multiplex immunofluorescence assay and a brightfield laboratory developed test

This study compares PD-L1 and CD8 quantification in NSCLC using a new ImmunoVUE™ multiplex immunofluorescence assay versus traditional brightfield IHC. Automated digital image analysis showed strong concordance with manual scoring, demonstrating that multiplex mIF can reliably measure PD-L1 and CD8 expression across tumor and immune compartments.