A novel method to minimize HIER-induced alterations on H&E staining in an integrated mIF-H&E workflow

Discover a new workflow that enables you to effectively combine H&E staining with mIF InSituPlex® assays and avoid the heat-induced epitope retrieval (HIER) steps that are the root cause of altered H&E staining. We present data showing co-registration of H&E and mIF images with sub-micron accuracy, enabling highly accurate molecular characterization and cellular phenotyping.