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Reagents & Consumables for MERFISH 2.0

Experience unmatched performance with high-quality reagents and proprietary consumables purpose-built for the MERSCOPE® Ultra Platform, delivering reproducible, high-resolution MERFISH® 2.0 insights from sample preparation through imaging.

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High-Quality Reagents for Reliable Spatial Genomics

Optimized with MERFISH® 2.0 Chemistry

Validated reagents built using MERFISH 2.0 chemistry to deliver consistent performance from sample preparation through imaging.

High-Throughput, High-Resolution Performance

Proprietary Vizgen slides feature alignment markings for precise tissue placement and a large imageable area, maximizing throughput and data yield.

Broad Sample Compatibility

Support for FFPE, fresh frozen, fixed frozen, and cultured cells ensures flexibility across tissue types and study designs.

Seamless Workflow Integration

All reagents and consumables are designed to integrate with Vizgen’s standardized workflow, ensuring reproducibility across labs.

Protein Co-Detection Ready

Compatible with Vizgen’s Protein Stain Kits, enabling simultaneous transcript and protein detection for multiomic spatial insights.

Trusted Vizgen Quality

Every reagent is quality-controlled and validated to deliver accurate, reproducible data.

Protein Co-Detection

Add multiomic spatial power to your MERFISH® 2.0 experiments.

Vizgen’s MERSCOPE® Protein Stain Kits enable simultaneous detection of RNA and proteins within the same tissue sample, revealing how gene expression translates to protein function. Protein co-detection supports up to 6-plex with the Protein Co-Detection Kits, or up to 9-plex through custom Vizgen Lab Services. The data integrates with the MERSCOPE® Vizualizer for exploration and initial visualization.

  • Multiomic measurements on a single sample
  • Up to 9-plex protein co-detection via Lab Services
  • Protein and membrane staining for cell boundaries
  • Streamlined analysis through Vizualizer or custom segmentation with the VPT

Cell Boundary Staining & Segmentation

Define cellular boundaries with precision for accurate spatial analysis.

Vizgen’s Cell Boundary Kit uses a combination of three protein stains to precisely delineate cell boundaries, enhancing segmentation accuracy and enabling robust single-cell analysis in the MERSCOPE® Vizualizer and Vizgen Post-Processing Tool (VPT). This allows confident identification of cell types and spatial relationships across all Vizgen datasets for precise boundaries.

  • Optimized for MERFISH 2.0 chemistry
  • Compatible with Vizualizer and VPT segmentation
  • Enables reproducible single-cell spatial analysis

MERFISH 2.0 Workflow

A unified, high-quality process for generating reproducible spatial data.

Vizgen’s standardized MERFISH® 2.0 workflow brings together every stage of the spatial genomics process—from tissue preparation and probe hybridization to high-resolution imaging on the MERSCOPE® Ultra Platform. Each step is validated for reproducibility, signal clarity, and compatibility across FFPE and frozen tissue types.

New to MERFISH 2.0? Learn how the chemistry advances sensitivity and throughput.

Explore Merfish 2.0 Technology

1. Preparation

Initial preparation workflows differ by sample type to ensure optimal compatibility with the MERFISH 2.0 assay. Each preserves the sample’s native spatial structure for downstream processing.

2. Anchoring

Target RNA molecules are primed for incorporation into the polyacrylamide gel matrix using MERFISH 2.0 anchoring chemistry, ensuring robust signal retention across multiple rounds of hybridization and imaging.

3. Gel Embedding

Samples are embedded in a gel that immobilizes transcripts and stabilizes tissue architecture, supporting high-fidelity hybridization and imaging.

4. Clearing

Cellular lipids and background fluorescence are removed through controlled clearing, producing optically transparent samples ideal for high-resolution MERFISH imaging.

5. Probe & Enhancer Hybridization

MERFISH 2.0 probe sets and enhancer oligos hybridize to target RNA molecules, enabling high-sensitivity, multiplexed spatial transcript detection across thousands of genes.

6. Imaging

Automated multi-round imaging on the MERSCOPE platform captures high-resolution spatial barcodes, generating comprehensive maps of gene expression within intact samples.

Proprietary Consumables Engineered for MERFISH 2.0 Precision

Product
Description

Proprietary Vizgen Slides

Available in standard and large formats, precision-marked slides are designed for accurate tissue placement and large imageable areas, enabling high-throughput MERFISH 2.0 imaging.

Flow Cells (FCX-S / FCX-L)

Interchangeable flow cells accommodate small to large tissue sections, allowing users to match imaging capacity to sample size for optimal throughput and sample economics.

Protein Stain Kits

Enable simultaneous transcript and protein detection, including cell boundary staining for accurate cell segmentation, available up to 9-plex through Vizgen Lab Services.

Sample Prep Reagents

Standardized reagents for frozen and FFPE samples, minimizing background and preserving signal quality.

Hybridization and Wash Buffers

Formulated for MERFISH 2.0 chemistry to ensure uniform hybridization and minimal variability across samples.

Frequently Asked Questions

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