InSituPlex® Multiplexed Immunofluorescence
Pathology-Grade Performance, Multiplex Power
Achieve high-resolution multiplexed immunofluorescence biomarker detection with the precision, flexibility, and reproducibility needed to accelerate translational and clinical research.
- Readily Configurable Multiplex Biomarker Panels
- High-Throughput, Quantitative Spatial Profiling
How InSituPlex® Works
Multiplexed DNA-Barcoded Immunofluorescence
InSituPlex® is a multiplexed immunofluorescence technology that delivers quantitative, pathology-grade biomarker detection with high specificity, sensitivity, and speed across FFPE and frozen samples.
- Direct Detection
- Isometric Amplification
- Sequential Imaging
InSituPlex® uses DNA-barcoded primary antibodies that bind directly to target proteins, eliminating the need for secondary antibodies and reducing background noise. Amplification chemistry then amplifies the fluorescence signal in proportion to the antigen abundance, ensuring a linear and quantitative measurement across both low- and high-expression targets.
Sequential imaging rounds decode multiple biomarkers within the same tissue section, generating precise spatial maps that preserve morphology and reveal co-localized expression patterns across cellular compartments. This streamlined process enables reliable, high-throughput imaging and reproducible quantification from every sample.
- Direct Signal Amplification – Signal intensity directly reflects antigen abundance for quantitative accuracy.
- True Signal Visualization – Co-localizes multiple biomarkers with high dynamic range.
- High Specificity – DNA-barcoded antibodies minimize background and cross-reactivity.
- Fast, Gentle Workflow – Simplified process preserves tissue morphology.
- Concordance with Pathology Gold Standard (DAB) – Proven alignment with traditional immunohistochemistry.
InSituPlex® Sets a New Standard in Multiplexed Immunofluorescence
High Dynamic Range
Differentiate subtle biological variations with high-sensitivity detection that captures both low- and high-abundance markers in a single assay, eliminating the need for additional assay optimization.
Expanded Signal-to-Background Ratio
Achieve clear, quantifiable results with optimized amplification chemistry that enhances accuracy and visual clarity across tissue types.
Configurable Multiplex Panels
Select from a validated menu of 30 biomarkers, or extend to 94 developed biomarkers, enabling complete flexibility in panel design.
Pre-Optimized Kits within Weeks
Accelerate research with plug-and-play assays that deliver consistent performance, shorter development timelines (custom panels within 8 weeks), and reproducible results.
Preserved Tissue Morphology
Maintain native tissue architecture with a fast and gentle workflow designed to protect precious clinical and translational samples for downstream multi-omic applications.
Scalable Whole-Slide Imaging
Process hundreds of samples per day, to scale up discovery, translational, and clinical research studies with pathology-grade precision and consistency.
Why Choose InSituPlex®?
With InSituPlex®, researchers can:
- Quantitatively detect multiple biomarkers in one tissue section with pathology-grade precision.
- Visualize co-localized markers across cells for advanced spatial phenotyping.
- Preserve tissue integrity while capturing subtle biological variations.
- Accelerate studies through fast, high-throughput workflows- over 3× faster than traditional methods.
- Customize validated or expanded panels with OmniVUE and U-VUE for flexible spatial profiling in multiplexed spatial proteomics studies.
More markers. More context. More insight.
Peer-Reviewed Publications
Trusted by researchers worldwide, InSituPlex is featured in peer-reviewed studies that demonstrate precision, reproducibility, and impact across spatial biology and multiplexed spatial proteomics research.
Explore InSituPlex® Resources
Access eBooks, white papers, posters, and blogs showcasing the latest advancements in multiplex immunofluorescence and spatial proteomics with InSituPlex®.
EBOOK
Co-detection of Protein and RNA biomarkers on FFPE tissue section using InSituPlex® Technology
Download the Ebook
POSTER
High-multiplex automated staining and scanning workflow with Ultivue InSituPlex technology and the Olympus SLIDEVIEW VS200 slide scanner
Download the Poster