smFISH Verification Technical Note


To generate the best data possible using our MERSCOPE Platform, researchers must ensure they are working with high-quality RNA samples. Unfortunately, RNA is easily degraded by RNases when samples are mishandled or treated in ways that do not specifically protect against RNA degradation. Differences in sample origin, procurement, shipping conditions, and tissue processing workflows all introduce variability in RNA quality. Maintaining an RNase-free environment, properly preserving tissue samples, and carefully sectioning tissue blocks can all help protect samples from RNA degradation.