Publications
Guiping Wang, Cheen-Euong Ang, Jean Fan, Andrew Wang, Jeffrey R. Moffitt, Xiaowei Zhuang
bioRxiv doi:10.1101/2020.12.07.414060 (2020)
Neurons are highly polarized cells with complex neurite morphology. Spatial organization and local translation of RNAs in dendrites and axons play an important role in many neuronal functions. Here we performed super-resolution spatial profiling of…
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Publications
Jun-Han Su, Pu Zheng, Seon S. Kinrot, Bogdan Bintu, Xiaowei Zhuang
Cell 182(6):1641-1659.e26 (2020)
The 3D organization of chromatin regulates many genome functions. Our understanding of 3D genome organization requires tools to directly visualize chromatin conformation in its native context. Here we report an imaging technology for visualizing chromatin…
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Publications
Meng Zhang, Stephen W. Eichhorn, Brian Zingg, Zizhen Yao, Hongkui Zeng, Hongwei Dong, Xiaowei Zhuang
bioRxiv doi:10.1101/2020.06.04.105700 (2020)
A mammalian brain is comprised of numerous cell types organized in an intricate manner to form functional neural circuits. Single-cell RNA sequencing provides a powerful approach to identify cell types based on their gene expression…
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Publications
Miao Liu, Yanfang Lu, Bing Yang, Yanbo Chen, Jonathan S. D. Radda, Mengwei Hu, Samuel G. Katz & Siyuan Wang
Nature Communications 11, 2907 (2020)
The three-dimensional architecture of the genome affects genomic functions. Multiple genome architectures at different length scales, including chromatin loops, domains, compartments, and lamina- and nucleolus-associated regions, have been discovered. However, how these structures are arranged…
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Publications
Chong Wanga,Tian Lua, George Emanuel, Hazen P. Babcock and Xiaowei Zhuang
PNAS 116 (22) 10842-10851 (2019)
Pooled-library CRISPR screening provides a powerful means to discover genetic factors involved in cellular processes in a high-throughput manner. However, the phenotypes accessible to pooled-library screening are limited. Complex phenotypes, such as cellular morphology and…
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Publications
Robert Foreman, Roy Wollman
Mol Syst Biol (2020)16:e9146
Gene expression variability in mammalian systems plays an important role in physiological and pathophysiological conditions. This variability can come from differential regulation related to cell state (extrinsic) and allele-specific transcriptional bursting (intrinsic). Yet, the relative…
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Publications
Chenglong Xia, Jean Fan, George Emanuel, Junjie Hao, and Xiaowei Zhuang
PNAS 116, 19490-19499 (2019)
The expression profiles and spatial distributions of RNAs regulate many cellular functions. Image-based transcriptomic approaches provide powerful means to measure both expression and spatial information of RNAs in individual cells within their native environment. Among…
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Publications
Chenglong Xia, Hazen P. Babcock, Jeffrey R. Moffitt & Xiaowei Zhuang
Scientific Reports 9, 7721 (2019)
Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules…
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Publications
Jeffrey R. Moffitt, Dhananjay Bambah-Mukku, Stephen W. Eichhorn, Eric Vaughn, Karthik Shekhar, Julio D. Perez, Nimrod D. Rubinstein, Junjie Hao, Aviv Regev, Catherine Dulac, Xiaowei Zhuang
Science 362, 6416-eaau5324 (2018)
The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei—including the molecular identity, spatial organization, and function of distinct cell types—is poorly understood. Here, we developed an imaging-based in…
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Publications
Guiping Wang, Jeffrey R. Moffitt & Xiaowei Zhuang
Scientific Reports 8, 4847 (2018)
As an image-based single-cell transcriptomics approach, multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows hundreds to thousands of RNA species to be identified, counted and localized in individual cells while preserving the native spatial context of RNAs….
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