HCA Poster 2021: MERSCOPE Reveals the Transcriptional Organization of the Mouse Brain

We mapped 483 genes with MERSCOPETM across full mouse brain coronal slices. Enabled by MERFISH technology, MERSCOPE measures the position of individual molecules with single molecule resolution and high detection efficiency. For this demonstration, we constructed a panel of canonical cell type markers and nonsensory G-Protein coupled receptors (GPCRs) to spatially profile nonsensory GPCR expression across the brain with cellular context. Nonsensory GPCRs in the brain mediate signaling and may play vital roles behind brain ageing and neurodegenerative disorders. Our map demonstrates that MERSCOPE is a leading tool for molecular atlassing, enabling greater insight into healthy versus diseased tissue.