MERSCOPE Protein Stain Kit Workflow Antibody Selection Guidelines

  • Verify each antibody of interest does not have RNase contamination
  • Determine antibody specificity and concentration using immunofluorescence (IF) imaging.
  • Use the appropriate MERSCOPE Protein Stain Verification Kit to confirm by visual inspection that protein staining has acceptable antibody exposure before proceeding with a full MERSCOPE run.
  • Modify exposure by adjusting the antibody concentration and determining the correct laser settings.